Lab Safety

  Isolating Plasmid DNA https://www.youtube.com/watch?v=8xEDEJ0DHFA The purification of plasmid DNA from bacterial cells is an important step in the cloning workflow. During plasmid purification, bacterial cells are  lysed, freeing DNA and other cellular components from the cell wall .  This video gives brief, but a precise of description of what a plasmid is. The beginning of the video is them basically talking the viewer through what happens in the isolation of a plasmid then it goes on to a scientist doing the actual experiment. It was something to really enjoy watching.

  "How to pipette correctly – a short step-by-step introduction into proper pipetting"           https://www.youtube.com/watch?v=QGX490kuKjg A micropipette is a common yet an essential laboratory instrument  used to accurately and precisely transfer volumes of liquid in the microliter range . I enjoyed watching this video because it reminded me of how we learned to use micropipettes in the lab. This video shows exactly how to correctly use the instrument and gets up close so you can see what is exactly being pressed. Also I appreciated the fact that in parts of the video there is captions with some of the instructions that would be really important to pay attention to.

 https://www.youtube.com/watch?v=slY4qrnZIM8 This video was very helpful to understanding the process of colony transformation of E coli with Plasmid DNA. I like how at the very beginning the video takes time to show each one of the materials that will be used throughout the lab and tells the measurements of each one that is needed. I also really enjoy how they separate each of the steps making it easier for someone to backtrack if there is some sort of confusion.

 Serial Dilution video: https://www.youtube.com/watch?v=MCrNjHcfcpY The serial dilution method is  to estimate the concentration (number of colonies, organisms, bacteria, or viruses) of an unknown sample by counting the number of colonies cultured from serial dilutions of the sample , and then back track the measured counts to the unknown concentration I like this video because it's something that could teach someone at entry level biochemistry. It goes slow enough for you to comprehend what is going on and isn't so long and complex to where if you are confused on a step you don't have to replay the video multiple minutes back. So if anyone was to need a quick video to learn the essential basics of Serial dilution this is a video I would definitely recommend. LB agar video: https://www.youtube.com/watch?v=uQ_mqv7zngk Luria broth (LB) is a nutrient-rich media  commonly used to culture bacteria in the lab . The addition of agar to LB results in the formation of a ...

 On August 31st, 2021 we learned a great deal about what is considered to be safe and unsafe when conducting an experiment in the laboratory. We learned the importance of wearing leather shoes and that really stuck with me because I was under the impression before that all closed-toe shoes would be fine. Also through the video and discussion I learned the dangers of handling Dimethylmercury and the precautions and measures that should be taken to ensure safety is the first priority in the lab Lab safety Video: https://www.youtube.com/watch?v=HLaOJKD9iDg