Serial Dilution/Media Preparation/Extra credits

 Serial Dilution video: https://www.youtube.com/watch?v=MCrNjHcfcpY

The serial dilution method is to estimate the concentration (number of colonies, organisms, bacteria, or viruses) of an unknown sample by counting the number of colonies cultured from serial dilutions of the sample, and then back track the measured counts to the unknown concentrationI like this video because it's something that could teach someone at entry level biochemistry. It goes slow enough for you to comprehend what is going on and isn't so long and complex to where if you are confused on a step you don't have to replay the video multiple minutes back. So if anyone was to need a quick video to learn the essential basics of Serial dilution this is a video I would definitely recommend.


LB agar video: https://www.youtube.com/watch?v=uQ_mqv7zngk

Luria broth (LB) is a nutrient-rich media commonly used to culture bacteria in the lab. The addition of agar to LB results in the formation of a gel that bacteria can grow on, as they are unable to digest the agar but can gather nutrition from the LB within.This video is basically identical to what we did even though it was condensed to about fourteen minutes. I liked it because I could forsee what would happen next in each step. 


These two videos gives great instructions and great detail of what we learned in class it reiterates everything we was taught.

Comments

  1. Kiara I.September 23, 2021 at 1:51 PM

    The videos were very helpful and like you said can help someone understand entry level biochemistry. Good blog!

    ReplyDelete

Post a Comment

Popular posts from this blog

Micropipetting and Sterile Techniques

  "How to pipette correctly – a short step-by-step introduction into proper pipetting"           https://www.youtube.com/watch?v=QGX490kuKjg A micropipette is a common yet an essential laboratory instrument  used to accurately and precisely transfer volumes of liquid in the microliter range . I enjoyed watching this video because it reminded me of how we learned to use micropipettes in the lab. This video shows exactly how to correctly use the instrument and gets up close so you can see what is exactly being pressed. Also I appreciated the fact that in parts of the video there is captions with some of the instructions that would be really important to pay attention to.
Read more

Colony transformation of E coli with plasmid DNA

 https://www.youtube.com/watch?v=slY4qrnZIM8 This video was very helpful to understanding the process of colony transformation of E coli with Plasmid DNA. I like how at the very beginning the video takes time to show each one of the materials that will be used throughout the lab and tells the measurements of each one that is needed. I also really enjoy how they separate each of the steps making it easier for someone to backtrack if there is some sort of confusion.
Read more