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Showing posts from September, 2021

Colony transformation of E coli with plasmid DNA

 https://www.youtube.com/watch?v=slY4qrnZIM8 This video was very helpful to understanding the process of colony transformation of E coli with Plasmid DNA. I like how at the very beginning the video takes time to show each one of the materials that will be used throughout the lab and tells the measurements of each one that is needed. I also really enjoy how they separate each of the steps making it easier for someone to backtrack if there is some sort of confusion.

Serial Dilution/Media Preparation/Extra credits

 Serial Dilution video: https://www.youtube.com/watch?v=MCrNjHcfcpY The serial dilution method is  to estimate the concentration (number of colonies, organisms, bacteria, or viruses) of an unknown sample by counting the number of colonies cultured from serial dilutions of the sample , and then back track the measured counts to the unknown concentration I like this video because it's something that could teach someone at entry level biochemistry. It goes slow enough for you to comprehend what is going on and isn't so long and complex to where if you are confused on a step you don't have to replay the video multiple minutes back. So if anyone was to need a quick video to learn the essential basics of Serial dilution this is a video I would definitely recommend. LB agar video: https://www.youtube.com/watch?v=uQ_mqv7zngk Luria broth (LB) is a nutrient-rich media  commonly used to culture bacteria in the lab . The addition of agar to LB results in the formation of a ...

Lab Safety

 On August 31st, 2021 we learned a great deal about what is considered to be safe and unsafe when conducting an experiment in the laboratory. We learned the importance of wearing leather shoes and that really stuck with me because I was under the impression before that all closed-toe shoes would be fine. Also through the video and discussion I learned the dangers of handling Dimethylmercury and the precautions and measures that should be taken to ensure safety is the first priority in the lab Lab safety Video: https://www.youtube.com/watch?v=HLaOJKD9iDg